Determination of Gallic Acid,Quercetin and Kaempferol in Gongyanping Capsules by HPLC / 中国药师
China Pharmacist
; (12): 310-312, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-461067
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To establish an HPLC method for the determination of gallic acid,quercetin and kaempferol in Gongyanping capsules.Methods:
HPLC was applied with the chromatographic conditions as follows The chromatographic column was Agilent-SB C18 (250 mm × 4. 6 mm,5 μm) at 30℃; the mobile phrase was methanol-0. 3% phosphoric acid solution with gradient elution; the flow rate was 1. 0 ml·min-1.Results:
The linearity relationship of gallic acid, quercetin and kaempferol was within the range of 98.200-491.00 μg·ml-1(r=0.999 9), 7.520-37.600 μg·ml-1(r=0.999 9) and 4.940-24.700 μg·ml-1(r=0.999 9), re-spectively;the average recovery was 96. 74%(RSD=1. 33%), 98. 18%(RSD=1. 70%) and 97. 04%(RSD=1. 28%),respective-ly.Conclusion:
The method is simple, accurate and repeatable.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2015
Tipo de documento:
Artigo