Construction and inhibitory effect of microRNA expression vector targeting survivin on proliferation of human colorectal carcinoma HT-29 cells / 国际肿瘤学杂志
Journal of International Oncology
; (12): 22-26, 2015.
Article
em Zh
| WPRIM
| ID: wpr-462573
Biblioteca responsável:
WPRO
ABSTRACT
Objective To construct microRNA (miRNA) expression vector targeting surviving,and to investigate its effect on transfected human colorectal carcinoma (HT-29) cell apoptosis and proliferation.Methods miRNA targeting survivin was synthesized and transfected HT-29 cells by lipofectin.HT-29 cells were cultured in the 6 orifices.The cultured cells were divided into control,liposome,negative control and positive control groups.Transient transfected cells were collected and the proliferation index and apoptosis rate of HT-29 cells were detected by flow cytometry.The expressions of survivin mRNA and protein were detected by RT-PCR and Western blot.Results The proliferation index and apoptosis rate of the positive control group were significantly higher compared with normal group,transfection group and mock-vehicle group (17.98% ± 2.35% vs 38.04% ±2.11% vs 36.73% ±2.51% vs 36.57% ±3.05%; t =20.05,P<0.01; t =18.75,P<0.01; t=18.59,P<0.01; 19.54% ±1.74% vs 3.13% ±0.29% vs 3.70% ±0.44% vs 3.61% ± 0.50% ; t =16.40,P < 0.01 ; t =15.84,P < 0.01 ; t =15.92,P < 0.01).Survivin mRNA and protein expression levels were specifically suppressed in transfected HT-29 cells (t =0.68,P <0.01 ; t =0.58,P < 0.01; t=0.61,P<0.01;t=0.64,P<0.01; t=0.62,P<0.01;t=0.67,P<0.01).Conclusion Survivin targeted silence can effectively decrease the expression of survivin mRNA and protein,induce colorectal carcinoma HT-29 cell apoptosis and inhibit cell proliferation.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of International Oncology
Ano de publicação:
2015
Tipo de documento:
Article