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Production and phenotype identification of specific expressed green fluorescent protein in male mice germ cells / 中国比较医学杂志
Article em Zh | WPRIM | ID: wpr-463223
Biblioteca responsável: WPRO
ABSTRACT
Objective The aim of this study is production of organ specific animal model for studying reproductive toxicity in mice.Methods F1 generation was gotten by mating the Ddx4 -cre transgenic male mice with the Rosa26mT/mG transgenic female mice.F1 offspring and its parents phenotype was screened by molecular biological, histopathological and in vivo imaging technology.Results At molecular level, specific DNA fragment was only found in testis of F1 offspring; At the organ level, the expression of green fluorescent protein could only be observed in testis of F1 offspring; Testicular frozen sections and sperm fluorescence observation showed that green fluorescent protein were mainly expressed in the germ cell lineage such as secondary spermatocyte and spermatocyte and spermatozoon.Conclusions The production of the mice with specific germ cell expressed green fluorescent protein by Cre/loxP recombination system were built successfully.
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Texto completo: 1 Base de dados: WPRIM Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Chinese Journal of Comparative Medicine Ano de publicação: 2015 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Chinese Journal of Comparative Medicine Ano de publicação: 2015 Tipo de documento: Article
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