Changes of RAGE mRNA and Protein Expression in Thyroid of Diabetic Mellitus Rats / 天津医药
Tianjin Medical Journal
; (12): 118-120, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-473133
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To investigate the expression of receptor for advanced glycation end products (RAGE)in the thyroid tissue of diabetes mellitus (DM)rats.Methods:
The rat model of DM was established by high-lipid diet and vena caudalis injection of streptozocin.The rats of DM models were then randomly divided into three groupsDM group(n=17),insulin intervention group(n=16)and aminoguanidine intervention group(n=16).Nine normal rats were taken as controls.Twelve weeks after the establishment of DM model,rats were sacrificed and the thyroid tissue was taken to determine RAGE mRNA and the protein expression with the method of RT-PCR and Western Blot.Results:
The levels of RAGE mRNA and protein expression were higher in the thyroid tissue of DM rats than those in controls (P<0.05).The levels of RAGE mRNA and protein expression were lower in the thyroid tissue of insulin intervention group and aminoguanidine intervention group than thor of DM group (P<0.05).Conclusion:
The levels of RAGE mRNA and protein expression were up-regulated in the thyroid tissue of DM rats,which can be restrained by controlling blood glucose and blocking the formation of advanced glycation end products.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Tianjin Medical Journal
Ano de publicação:
2010
Tipo de documento:
Artigo