Determination of the activity of cytochrome C by the chromogenic substrate method / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
; (6): 138-140, 2015.
Article
em Zh
| WPRIM
| ID: wpr-477199
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish the chromogenic substrate method to determine the activity of cytochrome C.Methods Used TMB as the chromogenic substrate, reacted at 37 ℃ for 15 min, generated the yellow products, and detected the absorbance at 450 nm.The experimental design method is the 4 ×4 parallel line quantitative analysis.ResuIts The activities of cytochrome C injection samples have been determined.The linear regression equation was Y=0.9875 X -1.0221,R2 =0.9996.The accuracy and repeatability were 1.1 % and 3.6 %.ConcIusion The chromogenic substrate method was simple operation, sensitive and can be used to determine the activity of cytochrome C.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Biochemical Pharmaceutics
Ano de publicação:
2015
Tipo de documento:
Article