Effects of Rhodioloside on brain tissue oxidative stress in streptozotocin-induced diabetic rats / 国际中医中药杂志

ABSTRACT

Objective To investigate the protection of Rhodioloside on brain tissue in diabetic rats. Methods The rats were randomly divided into six groups a normal control group, a model control group, a Rhodioloside high, medium and low dose group, and a positive control group(n=16). Except normal control group, the diabetic models were set up by intraperitoneal injecting STZ(60 mg/kg). And then, the Rhodioloside high, medium, low dose group were respectively given Rhodioloside solution(100、50、25 mg/kg) by intragastrical administration(ig), the positive control group was given metformin hydrochloride(25 mg/kg) by ig, the normal control group and positive control group were given normal saline(NS). Before the drug was given and 4, 8, 12 weeks after, the level of blood sugar was determined. And 12 weeks later, the body weight was detected; the activity of CPK, LDH and the level of T-AOC in serum were determined; the activity of SOD, GSH-Px, CAT and the content of MDA in brain tissue were determined; and the histopathological changes of the brain tissue was observed by HE staining.Results Compared with the model control group, the level of blood sugar (16.0 ± 0.9 mmol/Lvs. 18.0 ± 1.2 mmol/L) in Rhodioloside high dose group was significantly decreased (P0.05). The brain histopathological changes of Sal treated groups were significantly improved, while the treatment effect of Rhodioloside high dose group was the most significant.Conclusions Rhodioloside had dose-dependent protective effect on the brain tissue in diabetic rats, whose mechanism perhaps related to its effects on enhancing the activity of antioxidant enzymes, and reducing the damage of oxidative stress.