Application of visible loop-mediated isothermal amplification ( LAMP ) technologies in detecting Oncomelania infected with Schistosoma japonicum / 军事医学
Military Medical Sciences
; (12): 133-136, 2016.
Article
em Zh
| WPRIM
| ID: wpr-488353
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a simple and rapid detection technique for Oncomelania infected with Schistosoma japonicum(SJ), with high sensitivity and good specificity .Methods The gene fragment of SJ was amplified by PCR , and cloned into the T-vector to construct positive-reference.An isothermal nucleic acid amplification reaction system for detecting Oncomelania infected with SJ was set up , and its sensitivity was analyzed by detecting positive-reference diluted according to geometric proportion , and its specificity by detecting the genomic DNA of relative samples .Then, a corresponding means of purifying nucleic acid was designed to assemble a reagent detecting Oncomelania infected with SJ . This reagent was validated by detecting Oncomelania samples.Results The 213 bp amplified products were obtained and used to construct recombination T-vector for positive reference .An isothermal nucleic acid amplification reaction system was set up for detecting Oncomelania infected with SJ , and the amplification results could be simply determined by color change, with better sensitivity and specificity .The reagents for detecting Oncomelania infected with SJ were assembled , which could detect samples containing only 1% infected Oncomelania.Conclusion A visible detection method for Oncomelania infected with SJ is successfully established and validated .
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Military Medical Sciences
Ano de publicação:
2016
Tipo de documento:
Article