CO-029 mediates TNFα/TNFαR1 induced epithelial mesenchymal transition in intrahepatic cholangiocarcinoma / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 121-124, 2016.
Article
em Zh
| WPRIM
| ID: wpr-488638
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the role of transmembrane protein C0-029 in epithelial mesenchymal transition (EMT) induced by tumor necrosis factor alpha (TNFα) of intrahepatic cholangiocarcinoma (ICC) cells.Methods RT-PCR and Western blot were conducted to detect the CO-029 expression in ICC cells and tissues.The effect of CO-029 silencing by lenti-virus on EMT induced by TNFα was investigated.Western blot and mass spectrometry after immunoprecipitation were used to confirm whether TNFαR1 can directly or indirectly bind with CO-029 to form complexes in ICC.Results Differential expression of CO-029 was observed in ICC cells and tissues.The expression of CO-029 was significantly reduced by lenti-viral interference in ICC cells,resulting in the failure of TNFα to induce EMT.TNFαR1 in ICC could directly or indirectly form complexes with CO-029.Conclusion CO-029 mediates TNFα/TNFαR1 induced EMT in ICC,which might play an important role in the invasion and metastasis.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Hepatobiliary Surgery
Ano de publicação:
2016
Tipo de documento:
Article