Content Determination of Calceolarioside A in the Leaves of Fraxinus mandschurica by HPCE / 中国药房
China Pharmacy
; (12): 4709-4710, 2015.
Article
em Zh
| WPRIM
| ID: wpr-500890
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of calceolarioside A in the Leaves of Fraxi-nus mandschurica.METHODS:
HPCE was performed on the capillary of uncoated fused silica capillary,buffer solution of borate buffered saline(20 mmol/L,pH 9.3),separation voltage of 20 KV by pressure injection(injection pressure was 50 bar with 3 s), detection wavelength was 350 nm,and column temperature was 30 ℃.RESULTS:
The linear range of calceolarioside A was 50-250μg/ml(r=0.999 7);RSDs of precision,stability and reproducibility tests were less than 3%,recovery was 97.48%-102.08%(RSD=1.12,n=6).CONCLUSIONS:
The method is simple,good reproducibility,and can be used for the content determination of calceolarioside A in the Leaves of F. mandschurica.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2015
Tipo de documento:
Article