Uncertainty Evaluation on the Determination of 6-Methylthiopurine by LC-MS/MS / 中国药师
China Pharmacist
; (12): 1651-1655, 2016.
Article
em Zh
| WPRIM
| ID: wpr-504579
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To evaluate the uncertainty in 6-methylthiopurine (6-MMP) determination by LC-MS/MS.Methods:
The uncertainty sources during the whole process of 6-MMP determination were established and evaluated. The combined and expanded un-certainties were also calculated.Results:
The expanded uncertainty at low (2 995 pmol) and high (140 900 pmol) concentration of 6-MMP was 275. 6 pmol and 11 396 pmol, respectively (P=95%, k=2).Conclusion:
The uncertainty in 6-MMP determination by LC-MS/MS is mainly caused by recovery, matrix effect and sample preparation at low concentration, and by recovery and sample prep-aration at high concentration.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Article