The Effect of Angiotensin-(1-7) on the Angiotensin II-induced Proliferation of Rat Glomerular Mesangial Cells / 中国医师杂志

ABSTRACT

Objective To explore the effect of angiotensin-(1-7) on the angiotensin-II(Ang II) induced proliferation of cultured rat's glomerular mesangial cells(GMC). Methods Ang-(1-7) was used in the cultured rat's GMC treated by AngⅡ, the synthesis of DNA and protein in GMC was measured by incorporation of thymidine and leucine, respectively, and the proliferation of GMC was measured by crystal violet staining. Results Ang-(1-7) inhibited the synthesis of DNA and protein of cultured GMC treated by AngⅡ in a dose-dependent manner. Ang-(1-7) also reduced the number of GMC treated by AngⅡ.The effects of Ang-(1-7) could not be blocked by both -AngⅡ,a specific AngⅡAT1 receptor antagonist, and AngⅡAT_2 receptor antagonist PD123319. Conclusion Ang-(1-7) could inhibit the proliferation of cultured rat's GMC induced by AngⅡ. The effects of Ang-(1-7) were not mediated by AngⅡAT1 and AT2 receptors.