The effect and mechanism of caveolin-1 upregulation on the proliferation of MGC803 cell line / 中国医师杂志
Journal of Chinese Physician
; (12)2001.
Article
em Zh
| WPRIM
| ID: wpr-528542
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the effect of caveolin-1 gene expression on the proliferation of human gastric adenocarcinoma cells,and to explore the possibility for its future usage in gene therapy.Methods The full-length caveolin-1 gene was stably transfected into the MGC803 cell line by lipofectin.The Pcl neo vector was transfected at the same time as mock control.The expression of caveolin-1 was detected by Western blot in both the caveolin-1 gene transfected MGC803 cells and the controls.The cell cycle was analyzed by flow cytometry.Results After transfected with caveolin-1,MGC803 cells significantly up-regulated the expression of caveolin 1 and extended their doubling time.The cell proliferation was inhibited and the cell cycle was arrested in the G_0/G_1 phase.Conclusion Caveolin-1 can inhibit the proliferation of MGC803 cells and induce cell cycle arrest in G_0/G_1 phase.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Chinese Physician
Ano de publicação:
2001
Tipo de documento:
Article