Methylation and aberrant expression of Wnt antagonist SFRP2 in gastric cancer / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
em Zh
| WPRIM
| ID: wpr-532692
Biblioteca responsável:
WPRO
ABSTRACT
AIM:To determine the methylation and expression of Wnt antagonist secreted Frizzled-related protein-2(SFRP2) in gastric cancer and to explore the role of SFRP2 in gastric carcinogenesis.METHODS:Methylation status of SFRP2 was detected by methylation-specific PCR(MSP).Real-time PCR was used to determine the expression of SFRP2 in gastric cancers and matched cancer adjacent normal tissues.SFRP2 expression in gastric cancer cell lines was determined by reverse-transcriptional PCR(RT-PCR) and Western blotting.RESULTS:SFRP2 methylation was found in 26(65%) gastric cancers and in 3(7.5%) matched cancer adjacent normal tissues.The frequency of methylation for SFRP2 was significantly higher in gastric cancers than that in matched normal tissues(x2=28.614,P
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2000
Tipo de documento:
Article