Study on Establishment and Optimization of Preparation Method of Protoporphyrin Disodium from Non- anticoagulant Porcine Blood / 中国药房

ABSTRACT

OBJECTIVE: To establish and optimize the preparation method of protoporphyrin disodium (NAPP) from non-anticoagulant porcine blood.METHODS: Heme was prepared from non-anticoagulant porcine blood firstly.Ferrous sulfide was added into heme to prepare NAPP by saponification and esterification reaction.UV absorption spectrum and infrared spectrum were applied for qualitative test,and ultraviolet spectrophotometry was adopted for quantitative detection of NAPP.Orthogonal experiment was used to optimize the preparation method of NAPP and to determine the optimal parameters of the preparation process.RESULTS: The preparation method of NAPP from non-anticoagulant porcine blood was established.10.6 g heme could be obtained from per kilogram of non-anticoagulant porcine blood and 3.46 g NAPP could be obtained from per 5 g heme.The purity quotient of NAPP was 88.8%.The optimal parameters for the preparation of NAPP were as followed incremental amount of methanol vs.chloroform was 70 mL ∶ 120 mL in estrification process;Incremental amount of toluene vs.1 mol?L-1 sodium hydroxide-methanol solution was 50 mL ∶ 80 mL in saponification process.The sample was extracted by continuous therma reflux at 90 ℃ for 2.0 h.CONCLUSION: The established preparation method of NAPP from non-anticoagulant porcine blood is an ideal approach for the preparation of NAPP with simple operation,abundant raw material,short preparation cycle,low cost and high yield.