Preparation nucleic acid vaccine of brucella melitensis and its immune effect / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1999.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-536362
Biblioteca responsável:
WPRO
ABSTRACT
Abstract Objective:
To construct bmcella melitensis Br.melitensis genomic DNA expression library and study its immune effect.Meth-ods Hind Ⅲ digested Br.melitensis genomic DNA fragments were cloned into pSV-?-Gal plasmds.The recombinant plasmids were transformedinto JM109 host bacteria. After large-scale isolation, the recombinant plasmids were inoculated into the quadriceps muscle of mice. In the 9thday after third boost immunization .agglutination antibodies against Br. melitensis and lymphocyte mitogenic ability of mice were determined. Re-sults Expression library nucleic acid vaccine can induce the production of agglutination antibodies against Br. melitensis and stimulate the lym-phocyte porliferation primed by ConA.Conclusion:
The genomic DNA expression library may provide a new vaccine against Br. melitensis infec-tion.
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
1999
Tipo de documento:
Artigo