INSULIN ENHANCES GLUT4 TRANSLOCATION AND GLUCOSE UPTAKE IN ISCHEMIC CANINE MYOCARDIUM IN VIVO / 解放军医学杂志

ABSTRACT

To investigate whether insulin enhances canine myocardial GLUT4 translocation and glucose uptake during ischemia in vivo, physiological parameters of myocardial metabolism were measured with an autoanalyzer, and GLUT4 distribution was assessed by immunoblot analysis and immunofluorescence. The results showed insulin significantly increased ischemic myocardial sarcolemmal GLUT4 content, and it was associated with up regulation of myocardial glucose uptake. Insulin increased coronary arterio venous difference by 4 fold in ischemic region. It suggested that insulin enhances ischemic myocardial GLUT4 translocation and glucose uptake. Insulin might contribute to increase myocardial glucose uptake and utilization during ischemia.