CLONING OF HUMAN ANGIOPOIETIN-1 GENE AND CONSTRUCTION OF ITS EUKARYOTIC AND PROKARYOTIC EXPRESSION VECTORS / 解放军医学杂志
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-553528
Biblioteca responsável:
WPRO
ABSTRACT
Angiopoietin is a novel angiogenesis related molecule. To investigate its roles in tumor angiogenesis and metastasis,angiopoietin 1 gene was obtained from human placental tissue by reverse transcription polymerase chain reaction (RT PCR). The sense, antisense eukaryotic expression vector pcDNA3.1/hangiopoietin1(+), antisense eakaryotic expression vector pcDNA3.1/hangiopoietin1(-), and prokaryotic expression vector pRSETC hangiopoietin1 were successfully constructed by directional cloning the target gene into pcDNA3.1 V5 His C and pRSET C vectors, respectively. This lays good foundation for further study on the roles of angiopoietin 1 in angiogenesis in tumors of the digestive tract.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
1983
Tipo de documento:
Artigo