Screening of binding protein of hepatitis C virus NS4A by phage display from human liver cDNA library / 解放军医学杂志
Article
em Zh
| WPRIM
| ID: wpr-554634
Biblioteca responsável:
WPRO
ABSTRACT
Objective To screen the HCV NS4A binding protein. Methods By using HCV NS4A as a solidified selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, the positive plaques was amplified and then cloned into the pGEM-Teasy vector. Two positive plaques were chosen for DNA sequencing. Results The binding protein of HCV NS4A was identified as mitogen-activated protein kinase (MAPK)-activated protein kinase 5 (MAPKAPK5) by BLAST. Conclusion This approach provides a new way for the study of the pathogenic mechanism of HCV infection.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
1982
Tipo de documento:
Article