T-type calcium channel effects on RyR and IP_3R of sarcoplasmic reticulum in atrial myocytes during atrial fibrillation / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-555923
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WPRO
ABSTRACT
Objective To inquire into T-type calcium channel effects on RyR and IP 3R of sarcoplasmic reticulum in atrial myocytes during atrial fibrillation.Methods Ten dogs underwent continuous rapid atrial pacing (500 beats/min) to create persistent atrial fibrillation.In five rapidly-paced dogs,mibefradil dihydrochloride was given beginning 2 days after pacemaker implantation,continuing until the twenty-fourth week.A group of size-matched dogs (n=5) without given mibefradil was used as a pure atrial fibrillation group.Another group of size-matched dogs (n=5) without pacemaker implantation was used as a control group.Canine atrial myocytes isolated by enzymatic dissociation were used to study T-type Ca 2+ channel blocker effects on expression and function changes of RyR and IP 3R in atrial myocytes by confocal microscopy.Results RyR/IP 3R ratio (0.2965?0.01812) was markedly lower than that of control group (2.7043?0.2293),but there was no difference compared with that of atrial fibrillation group (0.2472?0.1355).Ca 2+transient of atrial myocytes was nremarkably changed (1.3031?0.1056)(P
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Practical Internal Medicine
Ano de publicação:
2003
Tipo de documento:
Article