Effect of up-regulated esp1 gene expression on apoptosis of A549 cells / 第三军医大学学报

ABSTRACT

Objective To construct an expression vector containing esp1 in order to observe the effect of esp1 gene on the apoptosis of A549 cells. Methods A conditional plasmid vector containing esp1 was constructed, and transfected into A549 cells. The expression level of esp1 was detected by fluorescence microscopy, the chromosomes and the apoptosis, proliferation were detected. Results Transfection of IRE-EGFP-esp1 into A549 cells reduced improper chromosome segregation, cell proliferation and increased the apoptosis of A549 cells. Conclusion Up-regulation of esp1 not only has inhibitory effects on the abnormal proliferation and chromatid separation of tumor cells, but induces the apoptosis of A549 cells.