Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-572873
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To investigate the feasibility of fast assay of vitamin C (VC) in body fluids by iodometry with UV absorbance detection.Methods:The sample was pretreated with iodoacetic acid to block sulfhydryl group.Protein was precipitated by trichloroacetic acid,and chromogen was extracted with chloromethylene.The change of absorbance after 30 s reaction of the pretreated sample with iodine was measured.Results:The assay of absorbance before and after 30 s reaction of the pretreated sample with iodine showed the variation coefficient within 5%,and the recovery of VC was above 90%.There was linear response of the absorbance change to the amount of VC from body fluids with slope consistent to that for iodine.Conclusion:This method may be desirable for fast assay of VC in body fluids.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Journal of Chongqing Medical University
Ano de publicação:
2003
Tipo de documento:
Article