Simultaneous determination of isofraxidin and schisandrin in Ciwujia Naoling (Oral) Liquid by HPLC / 中成药

ABSTRACT

AIM: To establish a method of simultaneously determining effective components in Ciwujia Naoling Oral Liquid by RP-HPLC. METHODS: Stationary phase Alltech C_(18) column,the mobile phase was methanol(A)-0.4% HAC-water(B) with linear gradient elution.Detection was performed at wavelength of 344 nm,254 nm respectively for isofraxidin and schisandrin. RESULTS: The two effective components could be detected by this method,and their contents could be determined.A good linearity of isofraxidin and schisandrin was in the range of 0.88-17.6 ?g/mL,0.76-15.2 ?g/mL respectively.The average recoveries were 100.64%,100.33% respectively,RSD were 1.07%,0.65% respectively. CONCLUSION: The method is simple,feasible and reproducible and can be used for the quality control of Ciwujia Naoling Oral Liquid.