Determination of Berberine Hydrochloride in Shaoyao Decoction by HPLC / 中国中医药信息杂志
Article
em Zh
| WPRIM
| ID: wpr-580671
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WPRO
ABSTRACT
Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol/L potassium dihydrogen phosphate-triethylamine (30∶70∶0.14) as mobile phase at a flow rate of 1.0 mL/min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07~1.40 ?g. The average recovery was 98.63% (n=6, RSD=1.21%). The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Ano de publicação:
2006
Tipo de documento:
Article