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Clonging and Expression of Recombinant Human Vasostatin' s Domain and Its Ability to Inhibit Angiogenesis in CAM / 中国肿瘤生物治疗杂志
Article em Zh | WPRIM | ID: wpr-584110
Biblioteca responsável: WPRO
ABSTRACT

Objective:

To clone and express the recombinant human Vasostatin120-180aa domain and to investigate its activity of inhibiting angiogenesis in CAM.

Methods:

After amplifying gene of human Vasostatin120-180aa domain, we sub-cloned it into pQE30 vector and expressed Vasostatin120-180aa domain by E. coli. We also tested its ability of inhibiting angiogenesis in CAM.

Results:

The total gene length of human Vasostatin120-180aa domain is 180 bp. Expressed by pQE30 system in E. coli and purified by IMAC, Vasostatin120-180aa was detected by SDS-PAGE, in which there is a positive band and molecular weight is about 8 kD.

Conclusions:

Recombinant human Vasostatin120-180aa could play effective role in anti-angiogenesis in CAM and it showed a dose dependent effect in some degree.
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Texto completo: 1 Base de dados: WPRIM Idioma: Zh Revista: Chinese Journal of Cancer Biotherapy Ano de publicação: 1995 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Idioma: Zh Revista: Chinese Journal of Cancer Biotherapy Ano de publicação: 1995 Tipo de documento: Article