Observation of ultraweak luminescence of human hepatocarcinoma HepG2 cell / 医学研究生学报
Journal of Medical Postgraduates
; (12)2004.
Article
em Zh
| WPRIM
| ID: wpr-585952
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To explore the characteristics of the ultraweak luminescence of human hepatocarcinoma cells(HepG2). Methods: The intensity of the ultraweak luminescence of HepG2 cells and human hepatocytes(QZG)was examined by an IFFM-D chemiluminescence instrument.The intensity of the ultraweak luminescence of HepG2 cells and human hepatocytes(QZG)were affected by different concentration of Luminol and H_(2)O_(2). Results: In 10~(-4)mol/l Luminol and 0.3% H_(2)O_(2),the intensity of the ultraweak luminescence of HepG2 cells was significantly higher than that of QZG cells(P)0.05). Conclusion:The intensity of the ultraweak luminescence of HepG2 cells is higher than that of QZG cells.The intensity of the ultraweak luminescence is affected by cell number and the concentration of Luminol and H_(2)O_(2).The ultraweak luminescence reflect functional status of tumor cells.It can become an sensitive index of physiology and metabolism of tumor cells.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2004
Tipo de documento:
Article