High expression of the L-Methionine ?-lyase gene in E. coli / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-591443
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To identify and highly express the L-Methionine ?-lyase gene from Trichomonas vaginalis(TVMGL).Methods: The TVMGL gene was cloned into pGEX 4T-2,the recombinant protein expressed in E.coli DH5?and confirmed by SDS-PAGE.Results: Denaturing SDS-PAGE analysis confirmed the predicted size of the fusion protein(GST-TVMGL,68 000) and showed high purity after subsequent affinity chromatography on Glutathione sepharose 4B.The target proteins amounted to 34% of the total bacteria proteins.Conclusion: The L-Methionine ?-lyase gene was highly expressed in E.coli.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Article