Drug Resistance and Genotype of Extended Spectrum ?-Lactamases and Plasmid AmpC Enzyme-producing Klebsiella pneumoniae / 中华医院感染学杂志
Chinese Journal of Nosocomiology
; (24)2006.
Article
em Zh
| WPRIM
| ID: wpr-592378
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE To explore the drug-resistance,the existing forms,genotype,and transfer ways of extended spectrum ?-lactamases(ESBLs) and plasmid AmpC enzyme in Klebsiella pneumoniae.METHODS The drug sensitivity of K.pneumoniae to 17 antibiotics was done by slip-diffusion and microdilution methods.The genotype of two enzymes was assessed by PCR and sequencing.The transfer ways of K.pneumoniae drug-resistant gene were identified by transconjugants-test.RESULTS The ESBLs were mainly produced in 55 cefoxitin resistant K.pneumonia strains.The major genotypes of ESBLs and plasmid AmpC nzyme were CTX-M,MIR and DHA.These genes could be transferred from clinical isolates to recipient bacteria.CONCLUSIONS ESBLs as well as AmpC enzymes are the most important resistance mechanism in K.pneumoniae.The resistance could be transferred through the bacterial conjugation.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Nosocomiology
Ano de publicação:
2006
Tipo de documento:
Article