Granulocyte-Colony Stimulating Factor Mobilizing Autologous Peripheral Blood Stem Cells Transplantation to Form Cardiac Myoid Cells and Angiogenesis / 中国康复理论与实践

ABSTRACT

Objective To study autologous peripheral blood stem cells (PBSCs) transplantation for cardiac myoid cells formation and angionesis after recombinant human granulocyte-colony stimulating factor (rhG-CSF) mobilizing. Methods The 60 white Japanese big-ear rabbits were divided into 3 groups, i.e. transplantation (T) mobiliztor (M) and control (C) groups, each group with 20 rabbits. Myocardial infarction (MI) model was developed by ligating the anterior descending coronary artery. G-CSFs were given continually for 7 d in T and M groups since 1 h after MI model development. Cell suspension which derived from the peripheral blood and labeled with BrdU which prepared 1 week ago were injected into infarction regions and borders, while in C groups only the same doses saline was injected. The survival and differentiation of the implanted cells were detected with histological analyses and capillary densities. Results BrdU positive cells which were taken on immature cardiac myoid cells were observed at infarcted areas in T group, which were detected as the Actin positively. HE stains showed that the structures of infarction regions were deranged in C group, but in T and M groups cell arrangements were arranged regularly. Meanwhile, there were a large amount of neogenesis capillaries. The capillaries densities were respectively (58.2 ± 11.5) and (52.3±6.0) per high-power field in T and M groups, while in C group was (21.6±4.9) (P＜0.01 ). In C group blue collagen fibers were much more than T and M group under Masson stains (P ＜0.01). In T and M groups the cardiac functions were much better than in the C group at the end of 4 weeks, especially ejection fraction were respectively (65.34±2.54)% and (63.40±2.84)% in M and T groups. In C group it was only (50.51 ±6.47)% (P＜0.01). Conclusion After G-CSF mobilizing the implanted PBSCs may survive and differentiate into cardiac myoid cells in infarcted areas and vicinities, at the same time promote neogenisis and improve cardiac function. It is significant that cell transplantation will treat the cardiac infarction in future.