The mechanism research of STIM1 in breast cancer cells / 实用医学杂志
The Journal of Practical Medicine
; (24): 1373-1376, 2017.
Article
em Zh
| WPRIM
| ID: wpr-619384
Biblioteca responsável:
WPRO
ABSTRACT
Objectives To explore the calcium signaling mechanism of STIM1 in breast cancer cells. Meth-ods After SiRNA interruption, Western blot and Transwell were used to measure protein expression of STIM1 and cell migration in MDA-MB-231 cells respectively. The relationship between STIM1 and SOCE calcium signaling were analysed by Laser confocal microscopy. Western blots were used to measure protein expression of FAK after si-lence STIM1. Results The numbers of cells without STIM1 were significantly lower than those cells with STIM1 by Transwell assay. STIM1 mediated SOCE in MDA-MB-231. Blocking SOCE might inhibite cells migration. Si-lence STIM1 did not affect the expression or activation of FAK in MDA-MB-231 cells. Conclusion STIM1 influ-ences cell migration through SOCE pathway in breast cancer cells, which is independent on the expression or activa-tion of FAK.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
The Journal of Practical Medicine
Ano de publicação:
2017
Tipo de documento:
Article