Using FAB and PYG in coordinating with PCR on culturing, isolating and identifying enterotoxigenic bacteroides fragilis

ABSTRACT

The result of the study of using FAB and PYG in isolation and identification of ETBF from fecal samples were as follows: FAB medium had more advantages as compared to PYG. Together with using FAB, the PCR method can differentiate and identity ETBF from the B. fragilis species. The detection limit of PCR was 103 CFU of bacteria per ml of fecal suspension. Among 27 B. fragilis trains, 66% of them were positive with primer pair GBF 201-210 by PCR. The findings showed that ETBF can be detected by culturing samples in FAB together with identification by PCR. More studies could be done for detecting ETBF from fecal samples, especially from diarrhea patients