Gene cloning of murine alpha-fetoprotein gene and construction of its eukaryotic expression vector and expression in CHO cells / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 392-5, 2003.
Article
em En
| WPRIM
| ID: wpr-634065
Biblioteca responsável:
WPRO
ABSTRACT
To clone the murine alpha-fetoprotein (AFP) gene, construct the eukaryotic expression vector of AFP and express in CHO cells, total RNA were extracted from Hepa 1-6 cells, and then the murine alpha-fetoprotein gene was amplified by RT-PCR and cloned into the eukaryotic expression vector pcDNA3.1. The recombinant of vector was identified by restriction enzyme analysis and sequencing. After transient transfection of CHO cells with the vector, Western blotting was used to detect the expression of AFP. It is concluded that the 1.8 kb murine alpha-fetoprotein gene was successfully cloned and its eukaryotic expression vector was successfully constructed.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Transfecção
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Alfa-Fetoproteínas
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Células CHO
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Clonagem Molecular
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DNA Complementar
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Células Eucarióticas
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Vetores Genéticos
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2003
Tipo de documento:
Article