Inhibitory Effect of Curcumin on H_2O_2-Induced Production of Nitric Oxide and Reactive Oxygen Species in Mouse Embryo-Fibroblast / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)1993.
Article
em Zh
| WPRIM
| ID: wpr-640378
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the effects of curcumin on H2O2-induced the production of nitric oxide(NO) and reactive oxygen species(ROS) in mouse embryo-fibroblast.Methods Macrophage were collected in abdominal cavity of 6-8 weeks Kunming mouse,cultured macrophage(2?108 L-1) were divided into control group and curcumin groups randomly.Macrophage in H2O2 group were added into a single bolus of H2O2(1 mmol?L-1) for 30 min,macrophage in curcumin group were preincubated with different concentration of curcumin for 2 h followed by a 30 min incubation with 1 mmol?L-1 H2O2 and macrophage in control group were added into the same volume(0.1 mL) of 9 g?L-1 so-dium chloride.Immunocytochemistry was used to measure the contents of inducible nitric-oxide synthase(iNOS),NO production was determined by Griess reactive,ROS was determined by DCFH-DA Fluorescence proe.Results The production of NO in control group was little.The production of NO in H2O2 group markly highter than that in control group(P
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Applied Clinical Pediatrics
Ano de publicação:
1993
Tipo de documento:
Article