Research on the promoting role of apelin-13 in proliferation, migration and capillary-like tube formation of RF/6A cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 1042-1046, 2017.
Article
em Zh
| WPRIM
| ID: wpr-641226
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To investigate the effects of apelin-13 on proliferation, migration and capillary-like tube formation of a monkey choroid / retinal endothelial cell line, RF/6A, to clarify whether apelin-13 could promote retinal angiogenesis in vitro.METHODS:
RF/6A cells in good conditions were administrated with DMSO (the control group), apelin-13 at 0.1μmol/L (low dose group) or apelin-13 at 1μmol/L (high dose group).Cell proliferation, migration and capillary-like tube formation were detected by using the MTT assay, scratch assay and matrigel assay, respectively, at 24h after plating the cells.RESULTS:
Cell proliferation was promoted in both low and high dose apelin-13 groups compared to the control cells (P<0.05);the cell migration distance of both apelin-13 groups was significantly greater than that of the control group (P<0.05);and the number of capillary-like tube structures of both apelin-13 groups was significantly larger than that of the control cells (P<0.05).In addition, cell proliferation, migration and tube formation increased as the concentration of apelin-13 increased.CONCLUSION:
Apelin-13 could obviously promote the angiogenesis capacity of RF/6A cells, suggesting that apelin-13 was an important pro-angiogenic factor in retinal endothelial cells.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
International Eye Science
Ano de publicação:
2017
Tipo de documento:
Article