Expression of junctional adhesion molecule-1 in human Corneal epithelium / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

AIM:To investigate the expression and distribution of junction adhesion molecule-1(JAM-1)in human corneal epithelium and compare with that of occludin.METHODS:The expression in RNAs of JAM-1 and occludin was revealed by RT-PCR and the presence of protein was analyzed by the FACS method.Double immunofluorescent staining was used to determine the tissue distribution of JSM-1 and occludin in human corneal epithelium.RESULTS:The expression of JAM-1 and occludin was found in cultured human corneal epithelial cells.The double immunofluorescent study showed positive staining for JAM-1 at cell borders in the entire epithelial layer,while relatively extensive staining was seen in the superficial layer,where it COexisted with the expression of ocdudin.CONCLUSION:JAM-1 was expressed in entire layer of human corneal epithelium encircling the cells.