Application of multiplex polymerase chain reaction to identify Brucella / 中国地方病学杂志
Chinese Journal of Endemiology
; (6): 496-499, 2013.
Article
em Zh
| WPRIM
| ID: wpr-642804
Biblioteca responsável:
WPRO
ABSTRACT
Objective To evaluate the effect of multiplex polymerase chain reaction(Multiple-PCR) in identification of Brucella strains.Methods Six standard Brucella strains (Brucella abortus,Brucella melitensis,Brucella suis,Brucella canis,Brucella ovis,Brucella neotomae) were used as positive controls and Escherichia coli O∶157 and Yersinia enterocolitica O∶9 were used as negative controls.A total of 29 Brucella strains were tested.Brucella strains were amplified by BCSP31-PCR and the species of Brucella with positive results were identified with Multiple-PCR method.Results The results of all 29 amplified Brucella strains were positive with BCSP31-PCR.The identified results of all Brucella strains were positive with Multiple-PCR,including 20 strains of Brucella melitensis,5 isolates of Brucella suis,3 strains of Brucella abortus and 1 strain of Brucella canis.Conclusion Multiple-PCR method is a rapid,specific,simple and low risk method for identification of Brucella species.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Endemiology
Ano de publicação:
2013
Tipo de documento:
Article