Proteomic Analysis of the Specific Protein Expression in Inverted Papilloma / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: Inverted papilloma is a benign tumor of nasal cavity and paranasal sinuses with a propensity for local invasiveness, recurrence, and malignant transformation. Proteomics is a powerful tool for protein analysis, providing valuable information on biochemical processes involved in diseases, monitoring of cellular processes, and characterizing the protein expression levels. We tried to find the proteins that are associated with pathophysiology of the inverted papilloma and mechanisms of the disease by proteomic approach. MATERIALS AND METHOD: Normal nasal mucosa and inverted papilloma tissue was obtained during augmentation rhinoplasty and endoscopic surgery, respectively. Total protein was isolated and separated into numerous spots by two-dimensional electrophoresis. Twenty four protein spots that were only detected in inverted papilloma were selected and subsequently analyzed with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). RESULTS: About 700 protein spots were detected. Selected spots were analyzed, and various proteins were identified. These include T-cell receptor beta chains, Ca2+ binding proteins, caltractin, calneuron, ras-related proteins, a rab-2b oncogene family, chloride intracellular channel proteins, tumor protein D53, and tumor necrosis factor precursors. CONCLUSION: We identified the proteins expressed in the inverted papilloma with proteomic approach. These proteins may help us in understanding the mechanisms of pathogenesis of inverted papilloma, and may be used as possible tumor markers.