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Extraction Optimization of Quercetin and Kaempferol from Lindera Aggregate Leaves by Response Surface Method / 中国药师
China Pharmacist ; (12): 1731-1736, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-658296
Biblioteca responsável: WPRO
ABSTRACT

Objective:

To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves.

Methods:

On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time.

Results:

The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest.

Conclusion:

The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo prognóstico Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2017 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo prognóstico Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2017 Tipo de documento: Artigo
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