Extraction Optimization of Quercetin and Kaempferol from Lindera Aggregate Leaves by Response Surface Method / 中国药师
China Pharmacist
; (12): 1731-1736, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-658296
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves.Methods:
On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time.Results:
The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest.Conclusion:
The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2017
Tipo de documento:
Artigo