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MiR-223-3p modulates megakaryocyte polyploidization by targeting MYH10 / 军事医学
Military Medical Sciences ; (12): 552-559, 2017.
Article em Zh | WPRIM | ID: wpr-658672
Biblioteca responsável: WPRO
ABSTRACT
Objective To investigate the effect of microRNA-223-3p (miR-223-3p) on megakaryocytic differentiation and maturation, and explore the potential mechanism .Methods The endogenous expression of miR-223-3p during megakaryocyte ( MK) differentiation was detected by real-time PCR.Flow cytometry further indicated that alteration of miR-223-3p in human cell lines exerted effects on MK differentiation and maturation .By performing integrative bioinformatic analysis, the potential miR-223-3p target gene, MYH10,was identified.Real-time PCR, luciferase reporter assay and flow cytometry revealed that MYH10 was a direct target of miR-223-3p.Results Endogenous expression of miR-223-3p was in-creased with the differentiation and maturation of MK .The expression of megakaryocytic surface markers CD41 and CD61 and the ploidy were significantly increased in K562 and Meg-01 cells after transfection with miR-223-3p mimics.The expression of MYH10 decreased with the increase in miR-223-3p.Using a luciferase reporter assay ,we demonstrated that MYH10 was a direct target of MiR-223-3p.Furthermore, direct downregulation of MYH10 promoted MK polyploidization . Conclusion MiR-223-3p might regulate the polyploidization of MK by targeting MYH10.
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Texto completo: 1 Base de dados: WPRIM Idioma: Zh Revista: Military Medical Sciences Ano de publicação: 2017 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Idioma: Zh Revista: Military Medical Sciences Ano de publicação: 2017 Tipo de documento: Article