Dynamic study of magnetic resonance spectrum of brain metabolism of rats after burn stress / 实用医学杂志

ABSTRACT

Objective Magnetic resonance spectroscopy(MRS)was applied to analyze the metabolism of the frontal lobe and caudate nucleus of rats underwent severe burn stress ,so as to improve the understanding of early brain damage caused by severe burn. Methods A total of 35 healthy male Sprague-dawley(SD)rats weighted 250 to 300 g were randomly divided into 7 groups,including the control group and 3,6,12,18,24 and 48 hour burn group post-burn stress,with 5 rats in each group. The modified standard rat burn model proposed by Walker and Manson was established with second-degree burn wound covering 20 percent of body surface. The rats in each group were examined by MRI at corresponding time points to observe change of MRS. The potential change of Cho/Cr and NAA/Cr of bilateral frontal lobe and caudate nucleus were recorded. Results (1)Compared with the con-trol group,the NAA/Cr of post-burn rats were significantly reduced,while Cho/Cr was significantly increased(P<0.01,respectively).(2)Significant differences in NAA/Cr,Cho/Cr values were observed among different sampling time points(P < 0.01,respectively).(3)The NAA/Cr value of the frontal lobe was significantly lower than cau-date nucleus (P < 0.01). The Cho/Cr value of frontal lobe was significantly higher than caudate nucleus (P <0.01). The NAA/Cr value of the left and right sides of brain were similar ,and the Cho/Cr value of the right brain was significantly higher than the,left side(P = 0.018). Conclusions MRS can detect the abnormality of burn stress-induced rat brain damage in the early stage ,with certain regularity.