Macrophage Migration Inhibitory Factor (MIF) Induced Stromal Cell-derived Factor 1 (SDF-1) Production Via Nuclear Factor KappaB (NF-kappaB) Signaling in Rheumatoid Arthritis Fibroblast Like Synoviocytes (RA-FLS)
Immune Network
; : 39-47, 2007.
Article
em Ko
| WPRIM
| ID: wpr-66399
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Stromal cell-derived factor (SDF)-1 is a potent chemoattractant for activated T cells into the inflamed Rheumatoid arthritis (RA) synovium. To determine the effect of macrophage migration inhibitory factor (MIF) on the production of SDF-1 in the inflamed RA synovium. METHODS: The expression of SDF-1 and MIF in RA and Osteoarthritis (OA) synovium was examined by immunohistochemical staining. The SDF-1 was quantified by RT-PCR and ELISA after RA fibroblast like synoviocyte (FLS) were treated with MIF in the presence and absence of inhibitors of intracellular signal molecules. The synovial fluid (SF) and serum levels of MIF and SDF-1 in RA, OA and healthy control were measured by ELISA. RESULTS: Expression of SDF-1 and MIF in synovium was higher in RA patients than in OA patients. The production of SDF-1 was enhanced in RA FLS by MIF stimulation. Such effect of MIF was blocked by the inhibitors of NF-kappaB. Concentrations of SDF-1 in the serum and SF were higher in RA patients than in OA patients and healthy control. SDF-1 and MIF was overexpressed in RA FLS, and MIF could up-regulate the production of SDF-1 in RA FLS via NF-kappaB- mediated pathways. CONCLUSION: These results suggest that an inhibition of interaction between MIF from T cells and SDF-1 of FLS may provide a new therapeutic approach in the treatment of RA.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Osteoartrite
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Artrite Reumatoide
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Líquido Sinovial
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Membrana Sinovial
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Ensaio de Imunoadsorção Enzimática
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Linfócitos T
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NF-kappa B
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Quimiocina CXCL12
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Fibroblastos
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Macrófagos
Limite:
Humans
Idioma:
Ko
Revista:
Immune Network
Ano de publicação:
2007
Tipo de documento:
Article