RSUME promotes apoptosis of pituitary adenoma cell AtT-20 in mice / 基础医学与临床

ABSTRACT

Objective To explore the expression and influence of RWD structure small ubiquitin modified enhan-cer (RSUME) and inhibitor of nuclear factor kappa B-alpha (IκB-α) and nuclear factor kappa B-1 (NF-κB1) in pituitary adenomas of mice.Methods Real-time fluorescent quantitative PCR ( q-PCR) was used to detect the level RSUME, IκB-α, NF-κB1 mRNA and Western blot was used to detect RSUME and the nucleoprotein of IκB-α, NF-κB1 changes in the level of protein , flow cytometry was used to detect cell apoptosis .Results In protein level, RSUME and IκB-αexpression reduced ( P<0.05 ) , but NF-κB1 raised after transfection ( P<0.05); The level of RSUME mRNA was obviously lower after transfection than before (P<0.05).The level of IκB-αand NF-κB1 mRNA was not significantly changed; Flow cytometry confirmed cell apoptosis rate increased significantly after transfection.Conclusions RSUME can promote apoptosis of pituitary adenoma cells in mice by NF-κB.