Differential proteomic analysis of total protein of ASMC stimulated by wild and mutant IL-13 / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2001.
Article
em Zh
| WPRIM
| ID: wpr-685360
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WPRO
ABSTRACT
Objective To analyze the differential proteomics of ASMC stimulated by wild IL-13 and mutant IL-13 and to investigate the relations of protein profiles of ASMC to asthma and possible targets for the treatment of bronchial asthma.Methods The total proteins of ASMC stimulated by wild IL-13 and mutant IL-13 were separated by immobilized pH gradient(IPG)-based 2-DE and the differentially expressed protein spots were identified by matrix assisted laser desorption-time of flight mass spectrometry(MALDI-TOF-MS). Results The 2-DE detected approximately(840?21)spots on wild IL-13 samples and(892?17)spots on mutant IL-13 samples(n=3)and(685?19)spots matched.Six significantly differential proteins were subjected to MALDI-TOF-MS analysis and three of them were identified as stathmin 1,Ribosomal protein p~0 and NADH dehydrogenase.Conclusions ASMCs stimulated by wild IL-13 and mutant IL-13 present different proteomic profiles that may shed some light on the mechanism for the asthma causing effect of wild IL-13 and mutant IL-13.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Laboratory Medicine
Ano de publicação:
2001
Tipo de documento:
Article