Protective Effect of Fingolimod on Renal Ischemia Reperfusion Injury Model Mice and Its Mechanism Study / 中国药房
China Pharmacy
; (12): 54-57, 2018.
Article
em Zh
| WPRIM
| ID: wpr-704518
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To study the protective effect of fingolimod on renal ischemia reperfusion injury (RIRI) model mice and its mechanism.METHODS:
A total of 60 mice were randomly divided into sham operation group,model group,fingolimod group (1 mg/kg) and fingolimod+wortmannin group [fingolimod 1 mg/kg+phosphatidylinositol 3-kinase (PI3K) specific blocker wortmarmin 1.4 mg/kg],with 15 mice in each group.Except for sham operation group,RIRI model was induced in other 3 groups,and those model mice were given relevant medicine via caudal vein at once 24 h before surgery.Serum of mice were collected in each group after 24 h perfusion.Serum levels of Scr and BUN were measured by automatic biochemical analyzer.The pathological changes of renal tissue were observed under light microscope.The protein expression of intercellular cell adhesion molecule-1 (ICAM-1),monocyte chemoattractant protein-1 (MCP-1) and phosphorylated protein kinase B (p-Akt) in renal tissue were measured by Western blot assay.RESULTS:
Compared with sham operation group,the serum levels of Scr and BUN in model group were increased significantly (P<0.01).Pathological changes were found in the kidney,and RIRI led to widespread renal tubular epithelial cell injury,apoptosis and inflammatory cells infiltration.The protein expression of ICAM-1 and MCP-1 in renal tissue were increased significantly (P<0.01),the protein expression of p-Akt was increased slightly (P>0.05).Compared with model group,other indexes of fingolimod group were improved significantly (P<0.01) except that the protein expression of p-Akt in renal tissue was increased significantly (P<0.01).Compared with fingolimod group,above indexes of fingolimod+wortmannin group were reversed (P<0.05 or P<0.01).CONCLUSIONS:
Fingolimod can obviously ameliorate renal injury induced by RIRI in mice,the mechanism of which may be associated with the activation of PI3K/Akt signaling pathway.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2018
Tipo de documento:
Article
País de publicação:
CHINA
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CN
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REPUBLIC OF CHINA