Simultaneous determination of eight constituents in Wuli Huichun Pills by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of arctiin,arctigenin,forsythoside A,narirutin,naringin,rhoifolin,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Wuli Huichun Pills (Saposhnikoviae Radix,Arctii Fructus,Forsythiae Fructus,etc.).METHODS The analysis of 70％ methanol extract of this drug was performed on a 25 ℃ thermostatic Agilent TC-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-0.4％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.55％-99.50％ with the RSDs of 0.54％-1.72％.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Wuli Huichun Pills.