Simultaneous determination of eight constituents in Wuli Huichun Pills by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12): 110-113, 2018.
Article
em Zh
| WPRIM
| ID: wpr-710164
Biblioteca responsável:
WPRO
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of arctiin,arctigenin,forsythoside A,narirutin,naringin,rhoifolin,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Wuli Huichun Pills (Saposhnikoviae Radix,Arctii Fructus,Forsythiae Fructus,etc.).METHODS The analysis of 70% methanol extract of this drug was performed on a 25 ℃ thermostatic Agilent TC-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-0.4% glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r > 0.999 0),whose average recoveries were 96.55%-99.50% with the RSDs of 0.54%-1.72%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Wuli Huichun Pills.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2018
Tipo de documento:
Article