Simultaneous determination of seven constituents in Supplemented Ermiao Granules by HPLC and establishment of fingerprints / 中成药
Chinese Traditional Patent Medicine
; (12): 341-346, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-710191
Biblioteca responsável:
WPRO
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of phellodendrine chloride,berberine hydrochloride,jatrorrhizine hydrochloride,(R,S)-goitrine,astilbin,hesperidin and atractylenolide Ⅲ in Supplemented Ermiao Granules (Phellodendri chinensis Cortex,Atractylodis Rhizoma,Smilacis glabrae Rhizoma,etc.) and to establish fingerprints.METHODS The analysis of 70% methanol extract of this drug was performed on a 35 ℃ thermostatic Amethyst C18-H column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.05 mol/L potassium dihydrogen phosphate-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,291 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r =1),whose average recoveries were 95.77%-110.36% with the RS-Ds of 0.06%-2.94%.There were twenty common peaks in the fingerprints of fourteen batches of samples with the similarities of more than 0.990,five of which (5-hydroxymethylfurfural,phellodendrine,astilbin,hesperidin and berberine hydrochloride) were identified.CONCLUSION This stable,reliable and reproducible method can be used for the quality control of Supplemented Ermiao Granules.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2018
Tipo de documento:
Artigo