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Capsaicin-Sensitive Sensory Nerves Indirectly Modulate Motor Function of the Urinary Bladder / 대한배뇨장애요실금학회지
Article em En | WPRIM | ID: wpr-715333
Biblioteca responsável: WPRO
ABSTRACT
PURPOSE: The urinary bladder (UB) is innervated by both sensory and autonomic nerves. Recent studies have shown that sensory neuropeptides induced contractions in the detrusor muscle. Therefore, in a mouse model, we investigated the presence of interactions between the submucosal sensory nerves and the autonomic nerves that regulate the motor function of the detrusor muscle. METHODS: UB samples from male C57BL/6 mice were isolated, cut into strips, and mounted in an organ bath. Dose-response curves to norepinephrine and phenylephrine were studied in UB strips with and without mucosa, and the effects of preincubation with a receptor antagonist and various drugs on relaxation were also studied using tissue bath myography. RESULTS: Phenylephrine-induced relaxation of the UB strips showed concentration-related effects. This relaxation appeared in both mucosa-intact and mucosa-denuded UB strips, and was significantly inhibited by lidocaine, silodosin, and guanethidine (an adrenergic neuronal blocker). Meanwhile, phenylephrine-induced relaxation was inhibited by pretreatment with propranolol and calcitonin gene-related peptide (CGRP)–depletory capsaicin in UB strips with and without mucosa. CONCLUSIONS: The present study suggests that phenylephrine activates the α-1A adrenergic receptor (AR) of the sensory nerve, and then activates capsaicin-sensitive sensory nerves to release an unknown substance that facilitates the release of norepinephrine from adrenergic nerves. Subsequently, norepinephrine stimulates β-ARs in the detrusor muscle in mice, leading to neurogenic relaxation of the UB. Further animal and human studies are required to prove this concept and to validate its clinical usefulness.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenilefrina / Propranolol / Relaxamento / Banhos / Bexiga Urinária / Neuropeptídeos / Capsaicina / Norepinefrina / Peptídeo Relacionado com Gene de Calcitonina / Receptores Adrenérgicos Tipo de estudo: Diagnostic_studies Limite: Animals / Humans / Male Idioma: En Revista: International Neurourology Journal Ano de publicação: 2018 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenilefrina / Propranolol / Relaxamento / Banhos / Bexiga Urinária / Neuropeptídeos / Capsaicina / Norepinefrina / Peptídeo Relacionado com Gene de Calcitonina / Receptores Adrenérgicos Tipo de estudo: Diagnostic_studies Limite: Animals / Humans / Male Idioma: En Revista: International Neurourology Journal Ano de publicação: 2018 Tipo de documento: Article