Impaired Nucleoporins Are Present in Sporadic Amyotrophic Lateral Sclerosis Motor Neurons that Exhibit Mislocalization of the 43-kDa TAR DNA-Binding Protein
Journal of Clinical Neurology
; : 62-67, 2019.
Article
em En
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| ID: wpr-719298
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND PURPOSE:
Disruption of nucleoporins has been reported in the motor neurons of patients with sporadic amyotrophic lateral sclerosis (sALS). However, the precise changes in the morphology of nucleoporins associated with the pathology of the 43-kDa TAR DNA-binding protein (TDP-43) in the disease process remain unknown. We investigated the expression of nucleoporins that constitute the nuclear pore complex (NPC) in spinal motor neurons that exhibit sALS in relation to TDP-43 pathology, which is a reliable neuropathological hallmark of sALS.METHODS:
Paraffin-embedded sections of the lumbar spinal cord were obtained for immunofluorescence analysis from seven control subjects and six sALS patients. Anti-TDP-43 antibody, anti-nucleoporin p62 (NUP62) antibody, and anti-karyopherin beta 1 (KPNB1) antibody were applied as primary antibodies, and then visualized using appropriate secondary antibodies. The sections were then examined under a fluorescence microscope.RESULTS:
NUP62 and KPNB1 immunoreactivity appeared as a smooth round rim bordering the nuclear margin in normal spinal motor neurons that exhibited nuclear TDP-43 immunoreactivity. sALS spinal motor neurons with apparent TDP-43 mislocalization demonstrated irregular, disrupted nuclear staining for NUP62 or KPNB1. Some atrophic sALS spinal motor neurons with TDP-43 mislocalization presented no NUP62 immunoreactivity.CONCLUSIONS:
Our findings suggest a close relationship between NPC alterations and TDP-43 pathology in the degenerative process of the motor neurons of sALS patients.Palavras-chave
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Medula Espinal
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Imunofluorescência
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Poro Nuclear
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Complexo de Proteínas Formadoras de Poros Nucleares
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Fluorescência
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Esclerose Lateral Amiotrófica
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Anticorpos
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Neurônios Motores
Limite:
Humans
Idioma:
En
Revista:
Journal of Clinical Neurology
Ano de publicação:
2019
Tipo de documento:
Article