The treatment effect of novel hGHRH homodimer to male infertility hamster
The Korean Journal of Physiology and Pharmacology
; : 637-647, 2018.
Article
em En
| WPRIM
| ID: wpr-727861
Biblioteca responsável:
WPRO
ABSTRACT
Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in reproduction. To study the treatment effect of Grin (a novel hGHRH homodimer), the infertility models of 85 male Chinese hamsters were established by intraperitoneally injecting 20 mg/kg of cyclophosphamide once in a week for 5 weeks and the treatment with Grin or human menopausal gonadotropin (hMG) as positive control was evaluated by performing a 3-week mating experiment. 2–8 mg/kg of Grin and 200 U/kg of hMG showed similar effect and different pathological characteristics. Compared to the single cyclophosphamide group (0%), the pregnancy rates (H-, M-, L-Grin 26.7, 30.8, 31.3%, and hMG 31.3%) showed significant difference, but there was no difference between the hMG and Grin groups. The single cyclophosphamide group presented loose tubules with pathologic vacuoles and significant TUNEL positive cells. Grin induced less weight of body or testis, compactly aligned tubules with little intra-lumens, whereas hMG caused more weight of body or testis, enlarging tubules with annular clearance. Grin presented a dose-dependent manner or cell differentiation-dependentincrease in testicular GHRH receptor, and did not impact the levels of blood and testicular GH, testosterone. Grin promotes fertility by proliferating and differentiating primitive cells through up-regulating testicular GHRH receptor without triggering GH secretion, which might solve the etiology of oligoasthenozoospermia.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Reprodução
/
Testículo
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Testosterona
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Vacúolos
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Hormônio Liberador de Hormônio do Crescimento
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Cricetulus
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Taxa de Gravidez
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Marcação In Situ das Extremidades Cortadas
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Ciclofosfamida
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Fertilidade
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2018
Tipo de documento:
Article