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Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3
Mycobiology ; : 102-105, 2008.
Article em En | WPRIM | ID: wpr-730099
Biblioteca responsável: WPRO
ABSTRACT
The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30degrees C for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC50 value of 64 mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Peptonas / Acetilcolinesterase / Leveduras / Concentração Inibidora 50 / Yarrowia / Glucose / Concentração de Íons de Hidrogênio Idioma: En Revista: Mycobiology Ano de publicação: 2008 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Peptonas / Acetilcolinesterase / Leveduras / Concentração Inibidora 50 / Yarrowia / Glucose / Concentração de Íons de Hidrogênio Idioma: En Revista: Mycobiology Ano de publicação: 2008 Tipo de documento: Article