Association of interleukin-10 gene-592C/A polymorphism with concentration of interleukin-10 in children with bronchial asthma / 中华实用儿科临床杂志
Chinese Journal of Applied Clinical Pediatrics
; (24): 261-264, 2013.
Article
em Zh
| WPRIM
| ID: wpr-732953
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WPRO
ABSTRACT
Objective To explore the polymorphism of-592C/A of IL-10 gene promoter region in children with bronchial asthma and its relationship with serum concentration of IL-10.Methods Ninety-two children with bronchial asthma and 92 healthy children were selected for study,polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used for the analysis of-592C/A of IL-10 promoter region polymorphism.The frequencies of genotypes of IL-10 gene-592 locus (CC,CA and AA) and alleles were accounted respectively,and x2 test was used to analyze the difference between the groups.Enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of the serum IL-10,and F test and q test were used for statistical analysis.Results Compared with the healthy control group,there were significant differences in-592C/A polymorphism of IL-10 gene in asthma group.The frequencies of AA genotypes (56.5%) and A allele (73.9%) in asthma group were higher than those (34.8%,58.7%)in the control group,there were significant differences(x2 =9.32,P < 0.01 ;x2 =8.87,P < 0.005,respectively).The individuals with AA genotype and A allele were 3.25 (95 % CI:1.28-8.28,P < 0.05) and 1.99 (95 % CI:1.28-3.08,P <0.01) times susceptible to asthma compared with CC genotype and C allele.The serum concentration of IL-10 in asthma group was significantly lower than that in healthy control group,whether in attacking-stage or remission-stage,and there were significant differences (all P < 0.01).The individuals with AA genotypes had lower serum IL-10 concentration than those with CC genotypes (P < 0.05) . Conclusions The IL-10 gene-592C/A polymorphism is different significantly between children with bronchial asthma and healthy ones,and this polymorphism influences the concentration of IL-10.The individuals with AA genotype have relatively lower IL-10 concentration,and A allele may be one of genetic susceptibility factor of bronchial asthma in children.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Applied Clinical Pediatrics
Ano de publicação:
2013
Tipo de documento:
Article